Greater Brisbane will enter a three-day lockdown at 5pm tonight after four new cases of locally transmitted coronavirus were recorded overnight.
Two cases are linked to the PA Hospital cluster, the remaining two do not yet have a known source of infection.
"Two are known cases of one of the previous people, then we have two under investigation. We believe one of them is a nurse from the PA hospital," Premier Annastacia Palaszczuk said.
Contact tracers are currently working with a man who was in Gladstone from March 25 to March 28 while infectious.
Masks will be compulsory for all public indoor settings across the state and gatherings have been limited to 30 people inside of homes outside the Greater Brisbane area.
Visitors to hospitals, aged care, prisons and disability facilities will have limited visitation and hospitality venues must seat all patrons in regional and rural Queensland.
Chief Health Officer Dr Jeannette Young said contact tracing is underway in Gladstone to assess if the region will face further restrictions.
"We need to find out what venues the gentleman went to while he was there before we can make that call," she said.
Anyone who has visited Brisbane since March 20 must adhere to Brisbane's lockdown measures.
Dr Young said she was happy to see testing rates improve, with 11,624 COVID tests conducted on 6130 people.
"We have really seen a massive increase in testing rates in Brisbane city and also in that surrounding area," she said.