MSF Sugar's Maryborough mill has crushed its last cane with the company announcing it was decommissioning the facility.
Instead, contracted sugar cane will be crushed elsewhere from 2021.
The decision comes after Rural Funds Management purchased 5409 hectares of MSF Sugar's cane growing land in the district earlier this year, with a view to replanting with macadamia nuts.
Canegrowers Maryborough chairman Jeff Atkinson said while the news was disappointing, an agreement between MSF and Isis Central Sugar Mill was advanced for the cane to instead be processed at Childers.
"MSF Sugar has a responsibility to ensure growers are not disadvantaged and that arrangements are made to satisfy its obligations under our current Cane Supply Agreement with the company," Mr Atkinson said.
"We remain confident that there is a bright future for growing sugar cane in the Maryborough region, and we will engage constructively to ensure that happens."