ReachTEL polling of 730 residents in the electorate, obtained by Fairfax Media, reveals One Nation candidate Sharon Lohse would receive 25.4 per cent of the first preference vote.
Katter's Australian Party candidate Robbie Radel would receive 10 per cent, while the LNP's Colin Boyce would receive 27.5 per cent, according to the poll taken on the night of November 8.
With One Nation and KAP signing off on a preference deal earlier this week, and the reintroduction of compulsory preferential voting, it could boost One Nation’s chances in the central Queensland electorate.
The seat of Callide has a margin of 9.8 per cent to the LNP but the impending departure of party elder Mr Seeney, and a resurgent One Nation, puts the seat into question.
Located in traditional Nationals territory, Callide has been in the hands of the Nationals or the merged Liberal National Party since ithe seat was created in 1950, and losing it would be a blow to the LNP.
Mr Seeney has held the seat since 1998, the same year One Nation won 11 seats in the Queensland Parliament.
In March, Mr Seeney announced he would retire at the next election to live a "more normal life".
Asked for their opinion of One Nation leader Pauline Hanson, 47.4 per cent of people living in Callide were favourable, compared with 21 per cent who were a fan of Opposition Leader Tim Nicholls.
In the electorate of Gympie, 30.4 per cent of residents said they would cast their first preference vote for One Nation’s Chelle Dobson, while incumbent LNP member Tony Perrett would receive 28.7 per cent.
Out of the 11.9 per cent of voters who were undecided, 16.7 per cent opted for the One Nation candidate, while 11.5 per cent said the LNP.
Gympie, which now has a margin of 7.6 per cent, fell to One Nation MP Elisa Roberts in 2001, before she became an independent, with the Nationals winning it back in 2006.
Asked for their opinion of Mr Nicholls, 19.3 per cent of people polled in Gympie were favourable, compared with 44.3 per cent for Senator Hanson, showing strong support for the One Nation leader.
There were 807 residents polled in the electorate of Gympie on the night of November 8.
Mr Nicholls has said there would be no coalition or shared ministry with One Nation and LNP preferences would be decided on a seat-by-seat basis, while Premier Annastacia Palaszczuk said Labor would put One Nation last and there would be no deals with the party.
The Queensland election will be held on November 25, and One Nation has been tipped to wrest several seats from the major parties.